Alcoholic fermentation and biomass production

Bioreactor in batch mode


GENERAL SPECIFICATIONS


  • 10 L stainless steel tank,double jacket.
  • Upper lid, electro-polished stainless steel 304,vapor injection for sterilization, cleaning hole,
    one exhaust gas equipped with a rupture disk, 3 quick fitting connections for: reagent, compressed air and substrate injection.
  • Stirring with variator and display of fluid level, Electro polished shaft equipped with two stainless steel 304
    Rushton turbine. Anti foam system on surface, 4 removable baffles.
  • Compressed air circuit,Stainless steel filter, sterilizable by vapor injection. Sparger for air introduction in the medium
  • Vapor circuit
    General isolating valve, relief valve for low pressure, general
    feeding solenoid valve, pressure gauge, and controlling vapor
    valve. Three ways valve to select either fermentation, or sterilization.Vapor circuitry for the sterilization, three way valve.
  • Cold water circuit
  • Vapor/ Water mixer.

Instrumentation

  • Pt 100 Ω temperature probe.
  • Pt 100 Ω temperature probe. double measurement.
  • One O2 electrode (sterilizable in situ).
  • One pH electrode (sterilizable in situ).
  • One Bourdon pressure gauge and oil bath.
  • Float flow meter PCV 2.
  • Float flow meter Glass.

 

Dim : 180 x 80 x 180 cm – 150kg
SS tubular framework 40 x 40mm

 

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Alcoholic fermentation process using beer yeasts

Study biomass growth

Study the influence of operating parameters on growth and metabolite production

 

Different sterilisation techniques

Injection of steam in the fermentation tank

Bactericidal solutions and autoclave treatment of ancillary systems

Microbiological sterility control protocol